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  • 1
    Electronic Resource
    Electronic Resource
    Male sterility caused by cytoplasm of Brassica oxyrrhina in B. campestris and B. juncea (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 285-287 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic male sterility ; Brassica oxyrrhina ; B. campestris ; B. juncea ; Alien gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Synthetic alloploid Brassica oxyrrhina (2n = 18, OO) x B. campestris (2n = 20, AA) was repeatedly backcrossed with B. campestris to place B. campestris nucleus in the cytoplasm of B. oxyrrhina. Alloplasmic plants, obtained in BC5 generation, were stably male sterile but mildly chlorotic during initial development. Synthetic alloploid B. oxyrrhina-campestris was also hybridized with B. juncea to transfer B. oxyrrhina cytoplasm. Segregation for green and chlorotic plants was observed in BC1 and BC2 generations. By selection, however, normal green male sterile B. juncea was obtained in BC3. Pollen abortion in both B. campestris and B. juncea is post-meiotic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225965
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chloroplast substitution overcomes leaf chlorosis in a Moricandia arvensis-based cytoplasmic male sterile Brassica juncea (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1179-1182 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica juncea ; Moricandia arvensis ; Cytoplasmic male sterility ; Chlorosis correction ; Cytoplasmic hybrids ; Chloroplast substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A male sterile Brassica juncea line based on Moricandia arvensis cytoplasm was developed previously by backcrossing the somatic hybrid M. arvensis+B. juncea, and the gene for restoring fertility was introgressed. The CMS line is very severely chlorotic because of the presence of alien chloroplasts and flowering is delayed by 30–40 days, making it unsuitable for the exploitation of heterosis. We have resorted to another cycle of protoplast fusion between green fertile B. juncea and chlorotic male sterile B. juncea, and developed green male-sterile plants. Molecular analysis revealed that in green male-sterile plants chloroplasts of M. arvensis origin were substituted by those from B. juncea, giving rise to intergeneric cytoplasmic hybrids with mitochondria of M. arvensis origin. With the development of dark-green male-sterile plants, the CMS fertility restoration system is suitable for the production of hybrid mustard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051007
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    Paper (German National Licenses)
    Fulltext
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