ISSN:
1432-2242
Keywords:
Key words Arabidopsis
;
Microsatellites
;
DNA markers
;
PCR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In this paper we show how rogue genotypes in the parental stocks or contaminants among the crossed progeny of Arabidopsis thaliana can be readily identified and excluded from the breeding process using microsatellite markers derived from a small quantity of intact leaf tissue which has been alkali-treated. This method is fast and cost effective as it does not require DNA extraction, is highly reliable, and is less damaging to small plants where only limited quantities of plant tissue are available. Furthermore, a large number of samples can be processed in 1 day, facilitating the identification process prior to selfing or crossing the plants. In addition, the procedure could potentially be automated since no centrifugation is required.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220051092
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