ISSN:
1617-4623
Keywords:
Escherichia coli
;
mutY
;
DNA mismatch repair
;
DNA polymerase
;
Repair tract
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Repair of heteroduplex DNA containing an A/G mismatch in a mutL background requires the Escherichia coli mutY gene function. The mutY-dependent in vitro repair of A/G mismatches is accompanied by repair DNA synthesis on the DNA strand bearing mispaired adenines. The size of the mufY-dependent repair tract was measured by the specific incorporation of α-[32P]dCTP into different restriction fragments of the repaired DNA. The repair tract is shorter than 12 nucleotides and longer than 5 nucleotides and is localized to the 3′ side of the mismatched adenine. This repair synthesis is carried out by DNA polymerase I.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00286698
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