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  • BSR  (1)
  • DNA vaccines  (1)
  • Emulsion  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Colloid & polymer science 105 (1997), S. 228-238 
    ISSN: 1435-1536
    Schlagwort(e): Emulsion ; amphiphilic polymer ; associative polymerstability ; rheology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Maschinenbau
    Notizen: Abstract Hydrophobically Modified (with alkylamide chain) linear Poly(acrylic acid) Sodium Salt (HMPAANa) of various degree of grafting (τ) and alkyl chain length (n) were synthesized and used as emulsifiers ofn-dodecane/water (NaNO3 10−3M) system. Stability and flow properties of the resultingn-dodecane in water emulsions (dispersed phase volume fraction=0.5) were investigated as a function ofτ (τ=0 to 10% in mol),n (n=12, 18) and polymer concentration (0–10%). It was clearly shown that viscosification (via an associative mechanism of HMPAANa) of the external phase of emulsions is a key factor to explain dispersions breakdown. However, a complete description of the phenomenon could not be achieved without considering the adsorption of the polymer at the liquid-liquid interface which depends closely onτ andn. More generally, the behavior of emulsions was found to be described adequately by the degree of hydrophobicity of HMPAANa's using a simple model conjugating the effects ofτ andn. As a matter of fact, the model was also found to provide a satisfactory description of HMPAANa's aqueous solutions as well, so that a good picture of emulsions properties can be deduced from the polymer behavior in water.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-6776
    Schlagwort(e): DNA vaccines ; hydrophobic interaction chromatography ; plasmid DNA ; rabies
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The purification of a DNA vaccine against rabies by hydrophobic interaction chromatography (HIC) using a Sepharose gel derivatised with 1,4-butanediol diglycidyl ether was scaled up 60 times. The purification profile was not affected by increased loadings (up to 15 mg DNA) and a product with a consistent quality was obtained. Fourteen mg of plasmid with an HPLC purity of 100% were obtained in one run, corresponding to a 95% yield.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1573-0778
    Schlagwort(e): BHK ; BSR ; human recombinant IL-2 ; rabies virus ; recombinant BHK ; serum-free medium ; surface-attached growth ; suspension culture ; Vero
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The presence of serum in cell culture raises safety problems for the production of biologicals, thus a new serum-free medium (MDSS2) was developed. The evaluation of this medium for the growth of different cell lines (BHK-21 C13, BSR and Vero) has shown that cells grew in this medium similarly to standard serum-containing medium, independently of the culture system used: in static (as monolayer) as well as in agitated systems (in suspension in spinner and perfusion reactors). BHK-21 and BSR cells grew as aggregate cultures and could proliferate in both static and agitated culture systems. Vero cells stayed attached to a substrate and proliferated equally in static and in agitated microcarrier-culture systems. The cell densities obtained with BHK-21 cells depended only on the culture system used. They ranged from 2–3×106 to 6–12×106 cells per ml for static batch and perfusion reactor cultures respectively. The cell concentration was 3 to 6 times higher than in classical cultures performed in serum-containing medium. The cell densities obtained with Vero cells were indistinguishable from those obtained in serum-containing medium, whatever the cell culture system used. These cell lines have been used for the production of rabies virus. With respect to BHK-21 and BSR, similar production rates of rabies glycoprotein have been found as in the standard roller bottle process. The production of rabies virus and of viral glycoprotein by Vero cells cultivated in serum-free medium was augmented 1.5-fold and 2.5-fold, respectively, when compared to serum-containing medium. A recombinant BHK-21 cell line, producing human IL-2, can also proliferate in MDSS2, after addition of insulin. The specific IL-2 production rate was augmented 3–4 fold in comparison to serum-containing medium. For the cells tested, the MDSS2 serum-free medium is a good growth and production medium. Its use for cultivating other cell lines and/or for the production of other biologicals is discussed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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