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  • 1
    Electronic Resource
    Electronic Resource
    Repetitive somatic embryogenesis in Medicago truncatula ssp. Narbonensis and M. truncatula Gaertn cv. Jemalong (1999)
    Springer
    Plant cell reports 18 (1999), S. 398-405 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsMedicago truncatula ; Repetitive somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Medicago truncatula ssp Narbonensis and four genotypes of M. truncatula Gaertn cv. Jemalong were tested for their somatic embryogenesis potential using a two-step protocol. In the first step, embryogenic callus was induced in folioles isolated from shoots grown in vitro and cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid and zeatin. In the second step, somatic embryos were allowed to develop from the induced callus in MS growth-regulator-free medium. Individual somatic embryos were then isolated and transferred again to growth regulator free medium where they formed secondary somatic embryos in repetitive cycles. Conversion of somatic embryos into plantlets was achieved by isolating late-torpedo-phase somatic embryos with distinct cotyledons and reculturing them onto MS growth regulator free medium. The system of repetitive somatic embryogenesis in M. truncatula described here represents a permanent source of embryogenic material that can be used for the genetic modification of this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050593
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    DNA puff C4 ofBradysia hygida (Diptera: Sciaridae) contains genes unequally amplified and differentially expressed during development (1993)
    Springer
    Chromosome research 1 (1993), S. 121-126 
    Details
    ISSN: 1573-6849
    Keywords: Developmental gene amplification ; DNA puff ; molecular organization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report here the isolation and characterization of a 2.3 kb genomicEcoRl fragment that co-localizes in the DNA puff C4 ofBradysia hygida with a 4 kbEcoRl fragment previously characterized as containing part of a gene amplified and expressed in the salivary gland at the time when puff C4 expands. Verification of the relative amount of DNA complementary to these two genomic fragments shows that they are unequally amplified in the salivary gland. The fragment containing part of the gene expressed when puff C4 expands amplifies about eight times more than the 2.3 kb fragment. This 2.3 kb fragment also carries sequences complementary to RNA species present in the gland in a period when puff C4 has already receded. Based on these data we discuss the nature of the DNA puff and the possible way in which amplification is occurring at these sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00710035
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    Paper (German National Licenses)
    Fulltext
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