ISSN:
1573-6849
Keywords:
Developmental gene amplification
;
DNA puff
;
molecular organization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We report here the isolation and characterization of a 2.3 kb genomicEcoRl fragment that co-localizes in the DNA puff C4 ofBradysia hygida with a 4 kbEcoRl fragment previously characterized as containing part of a gene amplified and expressed in the salivary gland at the time when puff C4 expands. Verification of the relative amount of DNA complementary to these two genomic fragments shows that they are unequally amplified in the salivary gland. The fragment containing part of the gene expressed when puff C4 expands amplifies about eight times more than the 2.3 kb fragment. This 2.3 kb fragment also carries sequences complementary to RNA species present in the gland in a period when puff C4 has already receded. Based on these data we discuss the nature of the DNA puff and the possible way in which amplification is occurring at these sites.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00710035
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