ISSN:
1432-0983
Keywords:
Key wordsPhanerochaete chrysosporium
;
Differential display
;
Cellulose-binding domain
;
Differential gene expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cellulose-binding domains (CBDs) are present in the majority of fungal cellulases studied to-date. This work describes the use of targeted differential display, employing degenerate primers designed to anneal to variants of a region conserved in fungal CBDs, each in combination with an oligo-dT primer, to PCR-amplify cDNA sequences containing regions coding for such domains from Phanerochaete chrysosporium. After growth on either Avicel or carboxymethyl cellulose (CMC), five distinct, abundantly expressed cDNA sequences were obtained. Two of these originated from transcripts of the previously characterised cbhI.1 and cbhI.2 genes, whereas three were from novel genes. One of the latter was isolated only after growth on CMC. No such sequences were obtained after growth on xylan, suggesting that the expression of sequences containing such regions is down-regulated on this substrate. The use of targeted differential display both for isolating novel sequences and for studying the expression of known genes within a family is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050310
Permalink
