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  • 1
    Electronic Resource
    Electronic Resource
    Sex determination in the dioecious Melandrium (1990)
    Springer
    Sexual plant reproduction 3 (1990), S. 179-186 
    Details
    ISSN: 1432-2145
    Keywords: Melandrium album ; Dioecious ; Haploids ; Tissue culture ; Sex chromosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Melandrium album (2n=24), a dioecious species with heteromorphic sex chromosomes (XY, males and XX, females), has a strong genetic commitment for sex determination. We report here a procedure for obtaining haploid plants from cultured anthers and show that genotype, pollen stage, cold treatment and certain culture media components are essential for a reproducible yield of embryos. Our procedure increased the number of responsive anthers and not the number of responsive microspores per anther. Most likely, our experimental system allows the recovery of “competent” microspores, and this on a medium containing either an auxin or a cytokinin. All of the 36 anther-derived plants tested expressed a female phenotypic sex instead of the theoretical one male ∶ one female ratio. When analysed cytologically, the plants exhibited the corresponding female genetic sex (one or two X chromosomes).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00205227
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    On the genetic control of the regeneration response in the dioecious Melandrium album (1992)
    Springer
    Sexual plant reproduction 5 (1992), S. 292-297 
    Details
    ISSN: 1432-2145
    Keywords: Melandrium ; Dioecious ; Sex determination ; Regeneration ; Protoplast culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Melandrium album (2n = 24), a dioecious species with heteromorphic sex chromosomes (XY, male; XX, female), has a strong genetic commitment for sex determination. So far the in vitro regeneration response in this species has appeared to be restricted to female plants. We report here a procedure for achieving active regeneration from explants and mesophyll protoplasts of selected female plants. Shoots were frequently induced in hormone-free media and in the presence of cytokinins or low concentrations (〈0.5 mg/l) of auxins. The in vitro conditions employed did not affect the sex expression of the regenerated plants. Crosses between selected female plants with high regeneration capacity and nonregenerating males indicated that the regeneration response is genetically controlled and can be transmitted sexually into male and female progenies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197381
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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