ISSN:
1612-1112
Keywords:
Displacement chromatography
;
Displacement TLC
;
Overloaded elution chromatography
;
Spacer displacement thin-layer chromatography
;
Spot profile
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Migration of spots in displacement development was investigated using a planar stationary phase. The effects of the presence or absence of the additive substances in the carrier were demonstrated when these compounds also played a role in the competition for the binding sites of the stationary phase; however, they were not members of the displacement train. In the case of displacement chromatography, compounds A+B are well separated but located very closely to each other. Using a spacer (C) in the sample, the peaks or spots of A and B are aparted by C and their virtual separation is increased. When compounds A+B were displaced by each other as well as by the displacer, the presence of the additive compound C in the carrier (carrier-spacer) can transfer an otherwise displaced component (A) into the so-called overloaded elution region of the chromatogram. At the same time the additive compound C does not unconditionally reduce the performance of displacement chromatography.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02260457
Permalink