ISSN:
1432-0568
Keywords:
Wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP)
;
Calcitonin gene-related peptide (CGRP)
;
Dorsal root ganglion Double-labeling method
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The rat shoulder joint capsule is innervated by thin sympathetic and sensory nerve fibers, most of which contain calcitonin gene-related peptide (CGRP). In order to establish the origin and distribution of CGRP-immunoreactive (IR) fibers, wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) was injected into the shoulder joints of rats via a dorsal surgical approach. After WGA-HRP injection, the cervico-thoracic dorsal root ganglia (DRG) were removed and processed using both HRP histochemistry and CGRP immunohistochemistry. In the C4 to C7 DRG, small to medium-sized neurons (20–40 μm) were labeled by this combined method. The number and size of the labeled neurons were measured in the cervical 4th–7th DRG. The number of double-labeled neurons was one quarter of the total number of HRP-labeled neurons and 1/20 of the CGRP-IR neurons. Most of the double-labeled cells were located in the C6 ganglion, and the mean number of double-labeled neurons was 13 at this level. The distribution and function of the CGRP-IR fibers in the rat shoulder joint capsule are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00304432
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