ISSN:
0935-6304
Keywords:
Capillary supercritical fluid chromatography
;
Large bore column SFC pumping system
;
Double-stage flow splitter
;
Flame photometric detection
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A commercially available instrument with an SFC pumping system suitable for wide bore columns (4.6 mm i.d.) has been modified for capillary supercritical fluid chromatography (CSFC) by incorporating a double-stage flow splitter. The first flow splitter was installed in front of the sample injection valve in order to avoid a high solute split ratio. The second splitter was mounted in the column oven so that the injected sample (0.2 μL) would be split to the capillary column. In order to perform pressure programmed elution, a pressure regulating system equipped with a gradient programmer has been used. Flame photometric detection was optimized for the analysis of organosulfur compounds by CSFC. In this study, detection limits were found to be 6-14 ng and the experimentally determined exponent (n value) varied from 1.721 to 1.984 depending on the compounds tested. Sulfur- and phosphorus-containing thermally labile pesticides can be chromatographed and selectively detected by using CSFC/FPD in either sulfur- or phosphorus mode, respectively.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240140411
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