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  • 1
    Electronic Resource
    Electronic Resource
    The isolation of the acid phosphatase-1 gene of Drosophila melanogaster and a chromosomal breakpoint inducing its position effect variegation (1990)
    Springer
    Molecular genetics and genomics 224 (1990), S. 49-56 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila melanogaster ; Acid phosphatase-1 ; Position effect variegation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Over 120 kb of contiguous genomic DNA sequence derived from the 99C–99D region of the Drosophila melanogaster third chromosome were isolated by molecular cloning. Sequences within this region required for the expression of the lysosomal gene-enzyme system acid phosphatase-1 (Acph-1) were identified by both P element-mediated germline transformation and transient expression and lie within a single 5 kb fragment. Acph-1 is encoded by a 2.1 kb poly(A)+ RNA transcript, which is expressed throughout development. Enzyme activity peaks also correlate with increases in RNA abundance. The ca-74 deletion, which exhibits position effect variegation at the Acph-1 gene (Frisardi and Maclntyre 1984), was also partially characterized. The variegating ca-74 breakpoint is located approximately 20 kb proximal to the Acph-1 gene. Results suggest that the heterochromatin at this breakpoint comprises highly repetitive or satellite DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259450
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and analysis of the chromosomal region 26A of Drosophila melanogaster (1991)
    Springer
    Molecular genetics and genomics 226 (1991), S. 241-249 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila melanogaster ; Chromosome walking ; Chromosome structure ; α-Gdph-β-Gal-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Region 26A of the second chromosome of Drosophila melanogaster has been extensively characterized at the genetic level. We report here the cloning of virtually the entire 26A region via a bidirectional chromosome walk. Deletion and translocation breakpoints in the 26A interval have been localized at the molecular level by both chromosomal in situ hybridization and Southern analysis. The locations of the genetically defined loci in this chromosomal region have also been correlated with transcriptional units mapped onto the DNA of the proximal region of the chromosomal walk. The position of the α-glycerophosphate dehydrogenase (α-Gpdh) gene in 26A5-7 has been confirmed and a putative transcriptional unit for the β-galactosidase-1 (β-Gal-1 gene has been identified in the 26A7-9 interval.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273609
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A genetic and molecular analysis of P-induced mutations at the glucose-6-phosphate dehydrogenase locus in Drosophila melanogaster (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 429-438 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila melanogaster ; Glucose-6-phosphate dehydrogenase ; P element associated mutation ; Chromosomal rearrangement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We examined P factor induced mutations of the Zw gene of Drosophila melanogaster in order to learn more about the site specificity of such mutations. Approximately 70000 chromosomes were screened using a powerful positive selection scheme. As only two mutants were discovered, Zw is a “cold spot” for transposable element insertion. One mutation involved a complex P element associated chromosomal rearrangement which was used to define the orientation of the gene with respect to the centromere of the X chromosome. The second mutation was either a simple, non-dysgenically induced point mutation or a very unstable insertion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259616
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    Paper (German National Licenses)
    Fulltext
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