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  • Bulk isolation  (1)
  • Dystrophic axons  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Morphological alterations of Purkinje cell axons and presynaptic terminals in organotypic cerebellar cultures exposed to ionizing irradiation (1981)
    Springer
    Acta neuropathologica 54 (1981), S. 301-310 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Dystrophic axons ; Ionizing irradiation ; Tissue culture ; Presynaptic terminals
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Ionizing irradiation induced marked morphological alterations in the Purkinje cell axons and axonic terminals in vitro. The myelinated segments demonstrated a very poor development of the myelin sheath. The axoplasm demonstrated various alterations of the mitochondria, and the smooth endoplasmic reticulum and the numerous elongated presynaptic terminals contained large aggregates of tubulovesicular structures and neurofilaments arranged in parallel and reticular array.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00697004
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Culture and cryopreservation of adult human oligodendrocytes and astrocytes (1984)
    Springer
    Acta neuropathologica 64 (1984), S. 172-175 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Adult human ; Oligodendrocyte ; Astrocyte ; Tissue culture ; Cryopreservation ; Bulk isolation ; Immunofluorescence microscopy ; Cell type-specific markers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Human adult oligodendrocytes were bulkisolated from the brains obtained at autopsy by the Percoll density gradient method and cultured in vitro for 2–28 days. Cultured human oligodendrocytes were then scraped from the dishes or coverslips, frozen at −70°C in the presence of 10% dimethyl sulfoxide as a cryoprotectorant, and then stored in liquid nitrogen tank. After the fast thawing, 55%–70% of human oligodendrocytes plus astrocytes were found to survive in the culture. Identification of cell types was established by using antibodies directed against galactocerebroside (oligodendrocyte) and glial fibrillary acidic protein (astrocyte).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00695582
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
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