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  • 1
    Electronic Resource
    Electronic Resource
    Surface characteristics of oocytes from juvenile mice as observed in the scanning electron microscope (1980)
    Springer
    Anatomy and embryology 161 (1980), S. 19-27 
    Details
    ISSN: 1432-0568
    Keywords: Surface characteristics ; Oocytes ; Mouse ; Scanning Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The in vivo and in vitro development of the zona pellucida as well as the vitelline membrane surface of oocytes isolated from juvenile mice aged between 8 and 30 days p.p. were investigated by scanning electron microscopy. In vivo a consistent development of the zona pellucida surface can be observed, namely the formation of a fibrous network like structure interspersed with numerous pores. After enzymatic removal of the zona pellucida the surface of the vitelline membrane was studied. In “normal” oocytes with intact germinal vesicles, microvilli were distributed over the entire oocyte surface in all age groups investigated. After resumption of meiosis in vitro a characteristic differentiation on the vitelline membrane occurs. A glabrous polar region appears in primary oocytes and a glabrous polar body in secondary oocytes. The same differentiation in surface organization could be observed in the in vivo precociously matured oocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304666
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cytogenetic investigations on development and degeneration of oocytes from juvenile mice (1980)
    Springer
    Anatomy and embryology 161 (1980), S. 9-18 
    Details
    ISSN: 1432-0568
    Keywords: Cytogenetics ; Oocytes ; Development ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Oocytes were isolated by mechanical disruption of the ovarian tissue of juvenile mice aged between 8 and 28 days p.p. Different types of oocytes could be distinguished by direct observation using an inverted light microscope. Their relative amount within the oocyte population isolated was determined in relation to age of the animals. The following types were isolated and examined: 1. “Normal” oocytes containing an intact germinal vesicle (that is, a nucleus in dictyate stage). In all age groups 55 to 83% of the oocytes were of this type. Their nuclear developmental stage was determined by studying their capacity to resume meiosis in culture as a function of age. 45% of the oocytes isolated from 15 day old mice were competent to resume meiosis. In mice aged 21 days more than 80% of the oocytes measuring about 80 μm in diameter resumed meiosis in vitro. No further increase in mean diameter or in the amount of competent oocytes could be found in older mice. 2. Degenerating oocytes. Five different types could be distinguished. a) “Atretic” oocytes present in all age groups investigated with pyknotic or disintegrating nuclei; b) “Precociously matured” oocytes first observed in mice aged 15 days containing normal diakinesis-and metaphase II-chromosomes; c) “Vacuolized” oocytes first isolated on day 18 having metaphase II-chromosomes scattered within the cytoplasm; d) “Fragmented” oocytes also first observed 18 days p.p. containing chromatin dissociated into micronuclei of different sizes within the cytoplasmic fragements; e) “Ghost” oocytes present in all age groups, characterized by the disintegration of the cytoplasm so that only the surrounding zona pellucida can be clearly seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304665
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Long-term cultivation of plasma cell leukemia cells and autologous lymphoblasts (LCL) in vitro: A comparative study (1978)
    Springer
    Annals of hematology 36 (1978), S. 331-338 
    Details
    ISSN: 1432-0584
    Keywords: Plasma cell leukemia ; LCL ; EBV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two long-term cell lines were established in vitro from the peripheral blood of a patient with plasma cell leukemia: one line with plasma cell proliferation, the other with lymphoblastoid cell proliferation (LCL). The 9-month-old plasma cell line showed the typical morphology of plasmoblasts. The cells neither had B-nor T-lymphocyte characteristics, were EBV negative, and showed aneuploidy with various marker chromosomes, including the 14 q+ marker. The cytogenetic findings indicate a monoclonal proliferation of the plasmacells. No tumor growth in thymusless nude mice could be induced upon intracranial inoculation with these cells. In contrast, the autologous LCL, cultured after addition of exogenous EBV, showed the characteristic markers of lymphoblastoid cells, with the typical morphology of pear and handmirror-shaped lymphoblasts, growing in clumps. They had C3- and Fc-receptors, surface-Ig, E-rosette-negativity, a diploid karyotype, and EBV dependent macromolecule synthesis. The lymphoblastoid cells produced intracranial tumors in nude mice in 8 out of 8 attempts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01000590
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    Paper (German National Licenses)
    Fulltext
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