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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Comparative clinical pathology 4 (1994), S. 30-33 
    ISSN: 1433-2981
    Keywords: Age ; ELISA ; IgG ; IgM ; Immunotoxicology ; Sprague-Dawley rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Routine determination of IgG and IgM levels is likely to be included in future international immunotoxicity assessment guidelines with the rat as the required animal species. Only a few historical data are available on these two parameters in the rat. We describe a quantitative ELISA sandwich method using commercially available purified rat immunoglobulin as standards and provide the normal range for IgG and IgM values in healthy untreated 10 ± 2 to 30 ± 3 weekold Sprague-Dawley rats. Our data indicate that both IgM and IgG levels increase gradually with age. In addition, we determined IgG values in rats hyperimmunised with protamine, lysozyme and casein.
    Type of Medium: Electronic Resource
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