ISSN:
1612-1112
Keywords:
Terbutaline
;
Plasma
;
Automated HPLC
;
Column-switching
;
Electrochemical detection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary An automated method based on liquid chromatography has been developed for the determination of terbutaline in human plasma in the range of 5–50 pmole·ml−1. The necessary sensitivity and selectivity was obtained by using electrochemical detection and a microprocessor-controlled column switching system. A combination of three columns was used: a C8 type for pre-separation, a C18 type for trapping and, for final separation, a strongly acidic ion exchanger. The accuracy of the method was examined by comparison with a method based on gas chromatography — mass spectrometry. The overall precision was ±3.5% and ±2.2% respectively at 5 and 50 pmole·ml−1. The total absolute recovery for terbutaline and internal standard at the above concentration levels were in the range 85–106%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02258934
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