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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 226 (1980), S. 85-92 
    ISSN: 1434-4726
    Keywords: Cholesteatom ; Knochenzerstörung ; Elektronenmikroskopie ; Cholesteatoma ; Bone destruction ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Biopsy specimens were obtained at ear surgery of patients with cholesteatoma otitis and were studied by electron microscopy. The osseous tissue decalcified in EDTA demonstrated degradation of collagen matrix as well as extensive degeneration of bone cells. The surface of the eroded bone was found to be in direct contact with the subepithelial connective tissue or the granulation, but not with the keratinizing squamous epithelium of matrix. These results indicate that the bone destruction by cholesteatoma is of non-osteoclastic type and lend support to the view that the keratinizing squamous epithelium do not participate in bone destruction. On the ultrastructural basis, the inflammatory process in the soft tissue adjacent to bone and extensive degeneration of bone cells are considered as cardinal factors for bone destruction in this disease.
    Notes: Zusammenfassung Die elektronenmikroskopische Untersuchung von Knochenbiopsien bei Cholesteatomoperationen zeigt Veränderungen der kollagenen Knochenmatrix sowie eine ausgedehnte Degeneration von Knochenzellen. Dabei findet sich die Oberfläche des veränderten Knochens in direktem Kontakt mit dem subepithelialen Bindegewebe oder mit Granulationen, nicht aber mit dem Plattenepithel selbst. Die Untersuchungen zeigen, daß entzündliche Prozesse in den dem Knochen benachbarten Bindegewebslagen vorwiegend für die Knochenzerstörung bei Cholesteatom verantwortlich sind.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 243 (1986), S. 269-273 
    ISSN: 1434-4726
    Keywords: Otitis media with effusion ; Electron microscopy ; Human temporal bones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural studies of the middle ear mucosa appear to be of significant value in better understanding the pathology of otitis media with effusion (OME). Our present study was undertaken in order to take advantage of the use of electron microscopy in investigating all areas of the middle ear mucosa. Tissues studied were obtained from the fresh postmortem temporal bones of three patients with OME and terminal head and neck malignancies. In the mucoid type of effusion (cases 1 and 2), goblet cells were seen to proliferate and secretory activity was greatly enhanced. In contrast, there was no evidence of secretory cell proliferation in the serous type of effusion. It was noteworthy that accumulated fluid was not homogeneous in the same ear, as exemplified by case 1, in which both mucoid and serous effusions were present. This occurrence was possibly the result of topographic diversity involving the secretory activity of the middle ear.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 129 (1985), S. 198-213 
    ISSN: 1615-6102
    Keywords: Electron microscopy ; Freeze-substitution ; Fungi ; Microsporum canis ; Microtubules ; Mitosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitosis in the dermatophyteMicrosporum canis was studied by freeze substitution and electron microscopy, and analyzed by three dimensional reconstruction from serial sections of the mitotic nuclei. The interphase nucleus has associated nucleus-associated organelle (NAO) on a portion of the outer surface of the nuclear envelope, subjacent to which there was dense intranuclear material. The NAO divided and separated on the envelope, and a spindle was formed. The spindle was composed mostly of microtubules extended between opposite NAOs. Pairing of kinetochores was observed in the spindle from an early stage of development, when chromosomes were not so condensed, and remained unchanged while chromosome condensation proceeded until metaphase. Before the completion of nuclear division, daughter nuclei were connected by a narrow spindle channel, and then the nucleolus, whose structure underwent minimal change during mitosis, was eliminated into the cytoplasm.
    Type of Medium: Electronic Resource
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