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  • 1
    ISSN: 1432-2242
    Keywords: Key words Stylosanthes ; Internal transcribed spacer ; DNA sequence ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The internal transcribed spacer (ITS) regions 1 and 2 of the ribosomal DNA from Stylosanthes guianensis CIAT 1283 and cv ‘Schofield’ were amplified by polymerase chain reaction using conserved ITS primers from the 18S, 5.8S and 26S ribosomal genes flanking those regions. The entire region of 683 bp long was cloned, and seven clones were sequenced. Comparison of the ITS spacer regions with published DNA sequences of other plant species revealed limited homology only; this was in contrast to their comparison with the 5.8S rDNA sequences. The ITS1 region of 45 S. guianensis accessions was amplified by PCR and sequenced on both strands using the conserved primers ITS2-ITS5. These sequences, ranging from 201 to 204 bp, were aligned to each other to assess intra-specific polymorphism. Within the S. guianensis (Aubl.) Sw. species complex, 11 DNA sequence types could be distinguished based on an insertion/deletion (indel) event and 15 single base-pair substitutions. In 1 of the S. guianensis types, two kinds of ITS1 sequence were observed in each individual, reminiscent of an incomplete homogenization of the repeat structure in this type. Polymorphisms in the sequence of the ITS1 region were used to define molecular markers for S. guianensis on the basis of PCR-restriction fragment length polymorphism and selective PCR.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 22 (1998), S. 1-27 
    ISSN: 0363-9061
    Keywords: expansive clay ; hydromechanics ; unsaturated soils ; nuclear waste ; in situ test ; Engineering ; Civil and Mechanical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: BACCHUS2 in situ isothermal wetting experiment has been analysed by means of a coupled flow-deformation approach. Backfill material, a mixture of Boom clay powder and high density pellets, has been extensively tested in the laboratory in order to determine its hydraulic and mechanical properties. Parameters of constitutive equations were derived from this experimental data base. Two mechanical constitutive models have been used in the simulation of the ‘in situ’ experiment: a state surface approach and an elastoplastic model. Calculations have shown several features of the hydration process which help to understand the behaviour of expansive clay barriers. Predictions using both models have been compared with each other and with actual measurement records. This has allowed a discussion of the comparative mertis of both approaches and the identification of some critical parameters of backfill behaviour. Overall agreement between calculations and field measurements is encouraging and shows the potential of the methods developed to model the behaviour of engineered clay barriers in the context of nuclear waste disposal. © 1998 by John Wiley & Sons, Ltd.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
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