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Digitale Medien

Electron-cytochemical localization of alkaline phosphatase to G cells of Necturus maculosus antrum (1989)

Rutten, Michael J. ; Garrison, Robert G. ; Moore, C. Diane ; [weitere]

Springer

Cell & tissue research 258 (1989), S. 555-561

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ISSN: 1432-0878

Schlagwort(e): Alkaline phosphatase ; Cytochemistry ; Endoplasmic reticulum, smooth ; Gastrin ; G cell ; Golgi complex ; Necturus maculosus (Urodela)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Electron-cytochemical localization of alkaline phosphatase activity was performed on G cells of Necturus maculosus antral mucosa. Alkaline phosphatase activity was localized to the nuclear membrane, the Golgi/endoplasmic reticulum, and the limiting membranes of G cell peptide-secretion vesicles. There was no specific localization of alkaline phosphatase activity to the plasma membrane. Treatment of the tissues with levamisole (an alkaline phosphatase inhibitor) did not markedly reduce the specific alkaline phosphatase activity. Specific lead deposition was reduced by removal of the substrate from the reaction mixture. The results from this study on N. maculosus G cells demonstrate that alkaline phosphatase activity can be found in a non-mammalian gastric endocrine cell and that specific activity was localized primarily to those intracellular structures involved with protein biosynthesis.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00218867

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Digitale Medien

Visioplasticity solution of deformation problems: Data handling procedures (1983)

Moore, C. ; Vickers, G. W. ; Dwivedi, S. N.

Chichester [u.a.] : Wiley-Blackwell

International Journal for Numerical Methods in Engineering 19 (1983), S. 257-269

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Details

ISSN: 0029-5981

Schlagwort(e): Engineering ; Engineering General

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Mathematik , Technik allgemein

Notizen: Procedures for smoothing experimentally determined displacement fields are presented such that dynamic, non-steady-state metalworking processes can be analysed using the visioplasticity method. The procedure establishes a rectangular grid in which strain, strain rate and stress values may optionally be obtained at all points on the grid. The condition of material continuity is automatically satisfied by constraints during polynomial smoothing in position and time, using the Gram-Schmidt orthogonalization technique. Examples are given of quasi-static and dynamic plane strain upsetting operations.

Zusätzliches Material: 11 Ill.