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The determination of phenol in urine by enzymatic hydrolysis/headspace gas chromatography (1981)

Adlard, E. R. ; Milne, C. B. ; Tindle, P. E.

Springer

Chromatographia 14 (1981), S. 507-509

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ISSN: 1612-1112

Keywords: Gas chromatography ; Headspace ; Phenol ; Urine ; Enzymatic hydrolysis

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary A new method has been developed for the determination of phenol in urine in which the phenol conjugates are hydrolysed enzymatically and the liberated phenol is analysed by headspace chromatography. The results compare favourably with those obtained by the method of van Haaften and Sie in which acid hydrolysis is carried out in a heated GC precolumn. The enzymatic hydrolysis headspace technique appears to be very reliable and does not suffer from the disadvantages of precolumn acid hydrolysis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02265629

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