ISSN:
1432-069X
Keywords:
Beta-adrenergic receptors
;
Epidermis
;
Keratinocytes
;
Catecholamines
;
Calcium
;
Psoriasis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Lesional psoriatic skin displays reduced responsiveness to Β-adrenergic stimulating agents. To elucidate whether the receptor protein itself is responsible for this, lesional and non-lesional psoritatic skin was investigated ex vivo for maximal Β-adrenergic binding density (B max) and Β-adrenergic binding affinity (K D). Epidermal crude membrane homogenates (ECMH) were prepared from split-thickness skin biopsies and saturated with the lipophilic Β-adrenergic antagonist (—)-125I-iodocyanopindolol (ICYP) as radioligand. Specific binding was saturable and Scatchard transformation of the binding data revealed a homogeneous class of Β-adrenergic receptors in all nine experiments. The maximal Β-adrenergic binding density was significantly less in lesional than in non-lesional psoriatic skin (B max=49.7 ± 7.2 fmol/mg protein vs. 67.1 ± 2.2 fmol/mg protein, n=9, P〈0.05). The binding affinity was similar in lesional and in non-lesional skin (K D=9.0 ± 1.5 pmol/l vs. 8.0 ± 0.9 pmol/l). These results could at least partially explain the reduced responsiveness of the Β-adrenergic system in lesional psoriatic skin seen after stimulation with Β-adrenergic agonists.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00371601
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