ZIB

Treffer pro Seite

Treffer 1 - 2 | 2 Treffer

Sortierung

Digitale Medien

Sarcomere length during contraction of isolated guinea-pig ventricular myocytes (1999)

Sasaki, N. ; Mitsuiye, T. ; Noma, A. ; [weitere]

Springer

Pflügers Archiv 437 (1999), S. 804-811

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1432-2013

Schlagwort(e): Key words Cardiac myocytes ; Excitation-contraction coupling ; Sarcomere length ; Twitch contraction

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract An improved method was developed for measuring sarcomere length (SML) during twitch contractions of single cardiac ventricular myocytes, using a charge-coupled photodiode array self-scanning at a rate of 1.5 ms/element. The average resting SML of 111 cells was 1.88±0.04 µm (mean ±SD). When contractions were triggered by action potentials under perforated-patch conditions, the time course of SML shortening closely followed changes in cell length. A large variation was observed in contraction time course between myocytes, some cells having a phasic component with a duration at 50% shortening (full-width at half-maximum; FWHM) of approximately 40 ms, while others shortened more slowly (FWHM of phasic component @100 ms). FWHM was highly correlated with relaxation half-time, but with neither action potential duration nor resting SML. The kinetics of slowly contracting cells could not be converted to the rapid type by using conditioning trains or applying isoprenaline. The steady-state SML/pCa relation in ventricular myocytes was measured by applying solutions of various pCa immediately after localized punctures of the surface membrane using a focal laser beam. The Hill coefficient, n H, was @4–5 and K 1/2@400–500 nM, but there was no evidence of two populations of cells with different Ca2+ sensitivities.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004240050849

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Digitale Medien

Enantiospecific Synthesis of 2,5-Disubstituted Piperidine Derivatives: Synthesis of (+)-Pseudoconhydrine (1997)

Dockner, Michael ; Sasaki, N. André ; Riche, Claude ; [weitere]

Weinheim : Wiley-Blackwell

Liebigs Annalen 1997 (1997), S. 1267-1272

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0947-3440

Schlagwort(e): Alkaloids ; Enantiospecific synthesis ; Chiral building blocks ; 2,5-Disubstituted piperidines ; (+)-Pseudoconhydrine ; Chemistry ; Organic Chemistry

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: A novel methodology for the synthesis of any one of the four stereoisomers of a 2,5-disubstituted piperidine in optically pure form is described, starting from readily available chiral building blocks 10 and 11 (or their antipodes). The utility of this approach is demonstrated in the total synthesis of (+)-pseudoconhydrine hydrochloride, the structure of which was confirmed by X-ray analysis.

Zusätzliches Material: 1 Ill.