ISSN:
1432-072X
Keywords:
Yeast
;
Saccharomyces cerevisiae
;
Killer toxin
;
Extracellular glycoprotein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr=16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00410734
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