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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 195 (1986), S. 117-122 
    ISSN: 1432-041X
    Keywords: Plasma membranes ; Amphibian embryogenesis ; Neural induction ; Xenopus laevis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasma membranes were isolated in high yield from Xenopus gastrulae by repeated sedimentation in discontinuous sucrose gradients. Most of the yolk was separated by lowspeed sedimentation before centrifugation on the discontinuous sucrose gradients. The isolation of plasma membranes was followed by covalent labelling of the surface of dissociated gastrula cells with diazoniobenzene sulphonate, by electron microscopy and the distribution of enzymatic markers. The isolated plasma membranes have a low neural inducing activity as compared to other cell constituents.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 192 (1983), S. 45-47 
    ISSN: 1432-041X
    Keywords: Neuralizing factor ; Transmission ; Mesoderm ; Extracellular material ; Triturus embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The neural plates of very early neurula stages of Triturus alpestris were removed, the material which is released from the extracellular space between mesoderm and neural plate to the medium in which the embryos were dissected was isolated and extracted with phenol. The protein isolated from the phenol layer showed neural inducting activity. Proteoglycans isolated from the aqueous layer did not show such inducing activity. These results together with previously published experiments (Wilhelm Roux's Arch 184: 285–299) suggest that a neuralizing factor which is released from the mesoderm acts on the inner surface of the overlying dorsal ectoderm.
    Type of Medium: Electronic Resource
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