ISSN:
1573-2568
Keywords:
FLUORESCENCE IN SITU HYBRIDIZATION
;
FLOW CYTOMETRY
;
HEPATOCELLULAR CARCINOMA
;
CHROMOSOME ABERRATION
;
DNA PLOIDY
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Conventional cytogenetic studies havedemonstrated frequent abnormalities of specificchromosomes in hepatocellular carcinoma, although thereare few reports examining the relationship betweenchromosomal aberrations and clinicopathologic features. Inthis study, numerical aberrations of chromosomes 16, 17,and 18 were examined by fluorescence in situhybridization using pericentromeric DNA probes in 20 cases of surgically removed hepatocellularcarcinoma. DNA ploidy analysis was also performed byflow cytometry. Numerical abnormalities of chromosomes16, 17, and 18 were found in 7 of 19 cases, 15 of 20 cases, and 12 of 20 cases, respectively. Gainand/or loss of more than one chromosome was detected in16 of 19 cases. However, aneuploidy was seen in only 9of 20 tumors by flow cytometry. The incidence of aneusomy 17 and 18 increased with tumor sizeand stage progression. Fluorescence in situhybridization analysis demonstrated that numericalchromosomal aberrations accumulated with tumorprogression in hepatocellular carcinoma.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018838731634
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