ISSN:
1432-2145
Keywords:
Cabbage
;
Female gametophyte irradiation
;
Aneuploid plants
;
Radiation (nuclear)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We examined the prospects for using the female gametophyte irradiation technique in cabbage to reduce the number of generations needed for cytoplasm transfer. Three different crosses were used with one nuclear and two cytoplasmic male-sterile lines as female parents. The doses applied ranged from 100 to 700 Gy. Differences between the genotypes were observed only in embryo and plant production with varying dose. Several plants derived from the various experiments exhibited one or two recessive paternal markers and an aneuploid number of chromosomes, which shows an irradiation-induced loss of maternal chromosomes. However, no androgenetic haploid plant was obtained. The aneuploid plants could sometimes be backcrossed, and in one case a shift in the segregation ratio towards the paternal (non-irradiated) phenotype was observed. We concluded that while the female gametophyte irradiation technique could reduce the number of generations necessary for cytoplasm transfer, the damage induced seems too restricted for this technique to be applied in cabbage breeding at present.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00197379
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