ISSN:
0741-0581
Keywords:
Liposome
;
Unilamellar vesicle
;
Hexagonal HII phase
;
Lipidic particle
;
Filipin
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
Model membrane systems are used extensively in all aspects of membrane research, and freeze-fracture is the preeminent procedure for directly visualizing local structure in these lipid dispersions. Here we describe in detail the formation of liposomes and how freeze-fracture is routinely employed as a complementary technique to biophysical and biochemical procedures in the characterization of multilamellar vesicles (most commonly known as liposomes) and unilamellar vesicles. Many preparative procedures exist for the formation of multi- and unilamellar vesicles. Examples of each system are given and their properties as well as freeze-fracture morphology are discussed. The detection of lipid-phase transitions is considered, in particular, with emphasis on the application of freeze-fracture to the study of lipid polymorphism. We briefly discuss the fracturing of apolar lipids which do not adopt bilayer structures but which can be stabilized into microemulsions by a phospholipid monolayer. Finally, a critical assessment is made of filipin as a morphological marker for cholesterol domains in the plane of the bilayer.
Additional Material:
11 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060130403
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