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  • Fingerprinting  (1)
  • Terminal inverted repeats  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of the termini of linear plasmids from Nectria haematococca and their use in construction of an autonomously replicating transformation vector (1989)
    Springer
    Current genetics 16 (1989), S. 187-194 
    Details
    ISSN: 1432-0983
    Keywords: Fusarium solani ; Mitochondrial plasmids ; Terminal inverted repeats ; Terminal proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondria of isolate FS37 from Nectria haematococca mating population I (Fusarium solani f. sp. cucurbitae) contain two linear plasmids, pFSCI and pFSC2, of 9.2 and 8.3 kbp, respectively. Evidence for a protein blocking the 5′ termini of these plasmids was obtained from exonuclease digestion experiments. A single protein band with an apparent Mr of 80 K was labeled when the DNA-protein complex of either plasmid was reacted with [125I] Bolton-Hunter reagent and then digested with DNase I. DNA sequence analysis of the termini of both plasmids revealed long inverted repeats of 1,211 by (pFSC1) and 1,027bp (pFSC2). No sequence similarity was found between the terminal inverted repeats (TIRs) of pFSC1 and pFSC2, nor was any similarity identified between the TIRs of the these plasmids and sequences of TIRs from other linear DNAs. A restriction fragment containing the TIR of pFSCI conferred autonomous replication when incorporated into an integrative transformation vector of Ustilago maydis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391476
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Organisation and molecular analysis of repeated DNA sequences in the rice blast fungus Magnaporthe grisea (1997)
    Springer
    Current genetics 31 (1997), S. 361-369 
    Details
    ISSN: 1432-0983
    Keywords: Key words Repeated DNA ; Rice blast ; Fingerprinting ; Transposon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of a previously described repeated DNA sequence present as a 1.3-kb PstI fragment in the genome of the rice blast fungus Magnaporthe grisea was analysed by carrying out DNA fingerprint analysis of 36 isolates including rice, non-rice and laboratory strains. The analysis of various higher-molecular-weight PstI fragments with homology to the 1.3-kb repeat revealed that these may arise predominantly from transposon insertions or point mutations. Analysis of a 5.1-kb derivative revealed both a point mutation at a PstI site and an insertion of a putative transposable element which caused an increase in molecular weight from 1.3 to 5.1 kb. Another repeat element of 1.4 kb was identified and found to exist in association with the 1.3-kb repeat. Both 1.3- and 1.4-kb elements were found to be parts of MGR583 (Hamer et al. 1989), a LINE-like element. These elements were present in a high copy number in all the rice and a majority of non-rice pathogens indicating that MGR583 is not a host-specific sequence as reported earlier. Our results suggest that repeated DNA elements in M. grisea have amplified independently of one another and further indicate that different isolates of M. grisea may have evolved from several distinct lines of origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050217
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    Paper (German National Licenses)
    Fulltext
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