ISSN:
1432-203X
Keywords:
Key wordsPolygonum hydropiper
;
Flavanol
;
Procyanidin
;
Cell suspension culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cultured Polygonum hydropiper cells maintained in Murashige and Skoog (MS) medium supplemented with 10–6 m 2,4-D, 10–6 m kinetin, 0.1% casamino acids and 3% sucrose were transferred to medium containing a higher concentration of calcium chloride (15 mm). The content of flavanols in the cells on the 6th day was approximately twice that of the control culture (31.9–60.7 mg/g dry wt). However, the contents of other secondary metabolites such as chlorogenic acid and gallic acid were not changed. The levels of flavanols in the culture medium remained unchanged throughout the 21-day culture period. Of the the inorganic components supplemented to the culture medium , only elevated levels of calcium chloride induced an increase in flavanol contents of the cells. The results indicated that the elevated concentration of calcium in the culture medium played an important role in activating the accumulation of flavanols.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050656
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