ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Peak overlap
;
Fluorescence detection
;
H-Point standard additions method
;
Water analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The H-Point Standard Additions Method (HPSAM) is proposed in order to resolve overlapping peaks in liquid chromatography by using a conventional fluorescence detector. The method uses as analytical signals the heights or the areas obtained at two previously selected emission wavelengths, and good results are obtained for highly overlapping peaks with highly overlapping fluorescence spectra. The principal benefits of the method are the ease of finding the required wavelengths, its insensitivity to changes in the retention time of the peak from one injection to another, and the possibility of using it in highly or only partially overlapping peaks. We have applied the method to the determination of phenol and cresols in water, resolving by the proposed method the overlapping peaks ofm- andp-cresol.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02290310
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