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  • 1
    Electronic Resource
    Electronic Resource
    A new biochemical marker for foot-specific cell differentiation in hydra (1985)
    Springer
    Development genes and evolution 194 (1985), S. 453-461 
    Details
    ISSN: 1432-041X
    Keywords: Hydra ; Peroxidase activity ; Foot cell differentiation ; Foot activator ; Foot inhibitor ; Head inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mucous cells in the basal disk of hydra contain a peroxidase-like enzyme allowing specific staining of these cells with substrates for peroxidases. The peroxidase activity provides an excellent marker for foot mucous cell, differentiation and was used to follow the reappearance of footspecific cells during foot regeneration after amputation. By choosing the appropriate either soluble or precipitable substrate the peroxidase reaction was used both for a qualitative and for a quantitative evaluation of foot-specific differentiation in hydra. For histological studies diaminobenzidien was found to be a suitable substrate which forms a dark brown precipitate within the cells containing the peroxidase activity. For a quantitative evaluation of foot regeneration the soluble substrate 2,2-azino-di(3-ethyl-benzthiazoline-sulfonic acid-6) ammonium salt was used which after reaction with the enzyme gives rise to a diffusible green reaction product the concentration of which can be measured by its specific absorption at 415 nm. Based on the diffusible enzyme product a new quantitative assay for foot regenration was developed and applied to confirm the effect and specificity of morphogenetic substances which either inhibit or activate foot or head regeneration in hydra.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00868146
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The head and the foot inhibitor from hydra are not dowex artefacts (1984)
    Springer
    Development genes and evolution 193 (1984), S. 117-118 
    Details
    ISSN: 1432-041X
    Keywords: Hydra ; Regeneration ; Head inhibitor ; Foot inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a recent publication in this journal (Berking 1983) it was claimed (1) that the head inhibitor we isolated from hydra is a Dowex artefact, (2) that a separate foot inhibitor does not exist in hydra and (3) that the only inhibitor that has so far been isolated from hydra is one which inhibits head and foot regeneration equally well. These statements are incorrect and require a response. In the following, I would like to summarise our evidence that the inhibitors isolated from hydra, including Berking's inhibitor, have different specificities for head and foot regeneration. In addition, I would like to show that none of our substances are Dowex artefacts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848640
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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