Electronic Resource
Springer
International journal of legal medicine
109 (1996), S. 216-217
ISSN:
1437-1596
Keywords:
MN blood group system
;
Genotyping
;
Forensic evidence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Law
Notes:
Abstract A novel method of human MN blood group genotyping is reported using the polymerase chain reaction. Genotyping is based on two base substitutions characteristic of M and N alleles in the 2nd exon of the glycophorin A gene. Using a newly designed primer trio, PCR products for M (255 bp) and N (270 bp) alleles are rapidly and simultaneously detected by a single PCR procedure and subsequent polyacrylamide gel electrophoresis. This method enables MN genotyping from not only minute but also degraded DNA samples.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01225522
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