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  • 1
    ISSN: 1432-0533
    Keywords: Free-living amebas ; Encephalitis ; Granuloma ; Acanthamoeba ; Naegleria ; “Mycotic aneurysm”
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Primary amebic meningoencephalitis and granulomatous amebic encephalitis are well recognized clinicopathological entities caused by free-living amebas. Associated arteritis and “mycotic aneurysms” with infiltration of intracranial arteries by lymphocytes, amebic trophozoites and cysts have not been previously reported. A 26-month-old girl had a 3-week history of encephalitis, characterized, initially, by vomiting and lowgrade fever. Subsequently, she developed ataxia, generalized weakness, lethargy, and esotropia. The first CSF showed 490 RBC/μl, 705 WBC/μl with 90% mononuclears. Her pupils reacted briskly to light. Moderate nuchal rigidity, nystagmus, fixed downward gaze, anisocoria, bilateral 6th nerve palsy, left arm monoparesis and left Babinski were present. CAT scan revealed slight symmetrical dilatation of anterior horns of lateral ventricles and an area of abnormal enhancement above the 3rd ventricle. She died 14 days after admission, 5 weeks after onset of symptoms. The brain showed focal necrotizing encephalopathy, involving thalami, cerebellum, brain stem, and cervical and upper thoracic spinal cord. Numerous free-living amebic trophozoites and cysts were present within a chronic granulomatous encephalitis. There were thrombosis of basilar, posterior cerebral, and vertebral arteries with profuse chronic panarteritis, fibrinoid necrosis, and mycotic aneurysms.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0533
    Keywords: Acanthamoeba ; Naegleria ; Meningoencephalitis ; Granulomatous ; Free-living amebas ; Cysts ; Trophozoites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Amebic Meningoencephalitis (AM) and Primary Amebic Meningoencephalitis (PAM) are infectious diseases essentially confined to the Central Nervous System (CNS) and caused by free-living amebas of the genusAcanthamoeba (A.) andNaegleria (N.) respectively. AM due toA. sp. (Acanthamoeba castellanii andAcanthamoeba culbertsoni) have been reported in chronically ill debilitated individuals, some of them under immunosuppressive therapy, or in immunologically impaired patients without a history of recent swimming in contrast to cases due toN.sp. which usually occurs in healthy, young individuals with a recent history of swimming in man-made lakes or heated swimming pools. AM due toA.sp. is characterized by a subacute or chronic granulomatous meningoencephalitis involving mainly the midbrain, basal areas of the temporal and occipital lobes and posterior fossa structures. CNS lesions in AM are perhaps secondary and the portal of entry in humans is probably from the lower respiratory tract, genitourinary system or skin reaching the CNS by hematogenous spread. The predominant host reaction is usually composed of lymphocytes, plasma cells, monocytes and multinucleated foreign body giant cells. Necrosis is moderate and hemorrhage scant or absent. Cysts as well as trophozoites may be seen within the CNS lesions. PAM is due toNaegleria fowleri and is characterized by an hemorrhagic necrotizing meningoencephalities with an acute inflammatory response. Only trophozoites are found in lesions. The portal of entry is through the olfactory neuroepithelium. CNS tissues fixed in formalin may be used for further identification and taxonomical classification of the causative protoza using immunofluorescent antibody techniques (IFAT) and electron microscopic methods.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0533
    Keywords: Acanthamoeba ; Amebic encephalitis ; Corticosteroids ; Free-living amebas ; Immunosuppression ; Intracranial mass ; Naegleria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Clinical and brain biopsy or autopsy findings in six patients with Granulomatous Amebic Encephalitis (GAE) due toAcanthamoeba sp. were characterized by focal neurological symptoms, increased intracranial pressure, and focal neuroradiological findings. Craniotomies were performed because of the diagnostic possibility of a mass lesion such as a brain tumor or abscess. In four patients, frozen sections demonstrated free-living amebas. GAE may present as an acute or subacute intracerebral mass lesion with signs and symptoms of focal brain disease and should be differentiated from viral, bacterial, fungal, and other protozoal encephalitides.
    Type of Medium: Electronic Resource
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