ISSN:
1432-072X
Keywords:
Malic acid
;
Fermentation
;
Saccharomyces bailii
;
Malic enzyme
;
Fumarase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract 1. The main pathway of the anaerobic metabolism of l-malate in Saccharomyces bailii is catalyzed by a l-malic enzyme. 2. The enzyme was purified more than 300-fold. During the purification procedure fumarase and pyruvate decarboxylase were removed completely, and malate dehydrogenase and oxalacetate decarboxylase were removed to a very large extent. 3. Manganese ions are not required for the reaction of malic enzyme of Saccharomyces bailii, but the activity of the enzyme is increased by manganese. 4. The reaction of l-malic enzyme proceeds with the coenzymes NAD and (to a lesser extent) NADP. 5. The K m-values of the malic enzyme of Saccharomyces bailii were 10 mM for l-malate and 0.1 mM for NAD. 6. A model based on the activity and substrate affinity of malic enzyme, the intracellular concentration of malate and phosphate, and its action on fumarase, is proposed to explain the complete anaerobic degradation of malate in Saccharomyces bailii as compared with the partial decomposition of malate in Saccharomyces cerevisiae.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00405891
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