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  • 1
    Electronic Resource
    Electronic Resource
    Disialoganglioside GD2 in human neuroectodermal tumor cell lines and gliomas (1991)
    Springer
    Acta neuropathologica 82 (1991), S. 45-54 
    Details
    ISSN: 1432-0533
    Keywords: GD2 ; Monoclonal antibodies ; Gliomas ; Ganglisides ; Medulloblastomas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Monoclonal antibodies (mAbs) recognizing the disialoganglioside II3(NeuAc)2GgOse3Cer (GD2) were produced by immunizing mice with the GD2-expressing neuroblastoma cell line LAN-1 and a prefusion boost with purified GD2 coupled to Salmonella minnesota. Two IgM mAbs were isolated which demonstrated high levels of reactivity (binding ratios in excess of 100) with GD2 by solid-phase radioimmunoassay and positivity in high-performance thin-layer chromatography (HPTLC) immunostain; only one (DMAb-20) was subsequently shown by analysis with a panel of defined ganglioside species to be specific for the minimum epitope of GD2, GalNAcβ1-4(NeuAcα2-8NeuAcα2-3)Gal-. DMAb-20 was used to evaluate the expression of GD2 by malignant glioma and medulloblastoma cell lines using cell surface radioimmunoassay, indirect membrane immunofluorescence, HPTLC immunostain, and densitometric analysis of extracted gangliosides from selected cell lines. Sixteen of 20 (80%) malignant glioma and 5 of 5 medulloblastoma cell lines reacted with DMAb-20; in agreement with previous studies, 5 of 5 neuroblastoma and 2 of 3 melanoma cell lines also reacted with DMAb-20. GD2 was proportionally increased in the glioma and medulloblastoma cell lines relative to levels in normal brain, as determined by densitometric analysis. In a phenotypic survey of malignant glioma biopsies, tumor cells in 24 of 30 (80%) cases stained positively with DMAb-20. Reactive astrocytes, both within and adjacent to tumors, were frequently intensely stained. Among the morphological variants of glioblastoma examined, the most intense staining with DMAb-20 was observed in neoplastic gemistocytes, with the weakest or absent staining in small cell glioblastomas. As GD2 is a commonly expressed surface antigen of gliomas and medulloblastomas, expression of which is retained in tissue culture, DMAb-20 will be useful in determining the functional role of GD2 in cell-cell interaction, adhesion, and invasion, and in defining altered growth control mechanisms of central nervous system neoplasms in in vitro models.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310922
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Phylogenetic relationships of the Magnoliaceae inferred from cpDNA matK sequences (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 925-930 
    Details
    ISSN: 1432-2242
    Keywords: Key words Magnoliaceae ; Molecular phylogeny ; Chloroplast DNA ; matK gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The coding region of the matK gene was sequenced to infer the phylogeny of the family Magnoliaceae. Phylogenetic analyses of 21 matK sequences representing ten genera of Magnoliaceae and three outgroups suggest relationships among both subfamilies and genera. Monophyly of the subfamily Liriodendroideae (the genus Liriodendron) and the subfamily Magnolioideae is strongly supported, respectively. Within the subfamily Magnolioideae, three clades are formed: (1) the genus Magnlietia, (2) the subgenus Magnolia, and (3) the subgenus Yulania, with the genera Michelia, Paramichelia, Tsoongiodendron, Alcimandra, Kmeria, Parakmeria and Manglietiastrum. However, the genus Magnolia is shown to be a polyphyletic group, and the genus Michelia a paraphyletic group. Relatively low sequence divergences are detected among genera of the the subfamily Magnolioideae, ranging from 0.14% to 1.70%, especially in the tribe Micheliinae (0.14–0.98%). Molecular evidence from matK sequence data suggests that the phylogenetic positions and the delimitation of the eight genera Magnolia, Michelia, Tsoongiodendron, Paramichelia, Alcimandra, Kmeria, Parakmeria and Manglietiastrum need to be reconsidered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051563
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Progress in the development of the tin PASS granule detector (1993)
    Springer
    Journal of low temperature physics 93 (1993), S. 461-466 
    Details
    ISSN: 1573-7357
    Keywords: 07 ; 85.25.-j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Progress in the development of a PASS granule detector is reported. Arrays with small tin spheres, with radius r⋍2µm, have been fabricated. The responses of tin PASS samples, with r⋍2 µm and r=8 µm, to low energy γ-radiation have been measured and analyzed. Also the performance of a multilayer PASS has been investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00693461
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    Paper (German National Licenses)
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