ISSN:
1432-203X
Keywords:
Key words Festuca arundinacea Schreb.
;
Festuca rubra L.
;
Transformation
;
Highly regenerative tissue
;
Transgene expression
;
Abbreviations2,4-D: 2,4-Dichlorophenoxyaceticacid
;
BAP: 6-Benzylaminopurine
;
PAT: Phosphinothricin acetyltransferase
;
GUS: β-Glucuronidase
;
GFP: Green fluorescent protein gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Highly regenerative tissues of tall fescue and red fescue produced from mature seed-derived embryogenic callus were induced and proliferated on medium containing 2,4-dichlorophenoxyacetic acid (4.5 or 9.0 μM), 6-benzylaminopurine (0, 0.044, 0.44 or 2.2 μM) and cupric sulfate (0.1 or 5.0 μM) under dim-light conditions (10 to 30 μE m–2 s–1, 16 h light). Tall fescue tissues were transformed with three plasmids containing the genes for hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar) and β-glucuronidase (uidA;gus), and red fescue with three plasmids containing hpt, uidA and a synthetic green fluorescent protein gene [sgfp(S65T)]. DNA from T0 plants of eight independently transformed lines from tall fescue and 11 from red fescue were analyzed by PCR and DNA blot hybridization. The co-expression frequency of all three transgenes [hpt/bar/uidA or hpt/uidA/sgfp(S65T)] in transgenic tall fescue and red fescue plants was 25–27%; for two transgenes [hpt/bar or hpt/uidA for tall fescue and hpt/uidA or hpt/sgfp(S65T) for red fescue], the co-expression frequency was 50–75%.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990000238
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