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  • 1
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 491-506 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The electrochemical activity of the individual chemicals in the nutrient medium for growth of Thiobacillus thiooxidans was studied along with the effect of the gases in equilibrium with their solutions. Several chemicals were active individually; however, the magnitudes, as measured by changes in half-cell potential, were less than that observed when T. thiooxidans was present in their composite mixture. Sterilized Skerman's mineral salts and the American Type Culture Collection (without ferrous sulfate) media were not sensitive electrochemically to changes from pure oxygen to nitrogen atmospheres. When T. thiooxidans was present in these media, the biological half-cell emf became sensitive to changes in the oxygen content of the atmosphere in equilibrium with the organism and nutrient. The ability of T. thiooxidans to make an electrochemical contribution, as registered by a platinum electrode, is substantiated further by these investigations.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 471-490 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The growth of Thiobacillus Thiooxidans, utilizing sulfur in three media, was studied by observing changes in halfcell emf, bacterial cell count, and acid production, all as a function of time. A comparison of the biological halfcell emf with comparable control halfcells reveals that T. thiooxidans makes an electrochemical contribution to halfcell voltage. A change from the more complex medium of Skerman's mineral salts to ATCC allowed a clearer delineation of the ability of T. thiooxidans to make an electrochemical contribution. Reproducible biological halfcell emf's were obtained when the ferrous sulfate was removed from the ATCC medium. One halfcell, consisting of T. thiooxidans utilizing sulfur in ATCC, was observed over a 111-day period. During this time, the initial halfcell voltage of -0.35 V. decreased to a value of -0.64 V. (hydrogen emf series). T. thiooxidans, in utilizing sulfur, produces only sulfate ion, thereby simplifying the identification of an electrochemical contribution during growth.
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1432-0878
    Schlagwort(e): Gametogenesis polychaete ; Oogenesis ; Peritoneum ; Nurse cell ; Vitellogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Oogenesis in Typosyllis pulchra begins with a short proliferative gonial phase. Gonial clusters, consisting of six to eight syncytial sibling cells, are surrounded by gonial peritoneum and are eventually liberated into the coelom. At about the same time as the onset of the coelomic phase, one gonial cell in each cluster differentiates, begins to accumulate yolk and increases in size, eventually to become a mature oocyte. Yolk is composed of lipid droplets and membranebound protein granules. The protein component appears to be at least partially synthesized by the oocyte although there are some signs of endocytosis. Nurse cells and peritoneal cells are apparently able to manufacture some protein that may also be utilized by the oocyte. The nurse cells maintain cytoplasmic continuity with the oocyte, and mitochondria and ribosomes are often seen within the intercellular bridges between oocyte and nurse cell. Immediately prior to spawning, oocytes undergo prematuration and cytoplasmic reorganization. Ultrastructural changes which occur during oocyte growth and maturation are described.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    ISSN: 1432-0878
    Schlagwort(e): Gametogenesis polychaete ; Stolonization ; Gonad ; Gonial cell ; Spermiogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Typosyllis pulchra reproduces by the production of three to four gamete-bearing stolons (schizogamy) during consecutive 30-day periods. Although gonads are found in a large number of segments, only those in the posterior-most segments produce gametes and become incorporated into the developing stolon. The more anterior gonads remain undifferentiated and probably sexually undetermined until they are needed in future stolonizations. Gonial cells, which will eventually become either male or female, are ultrastructurally identical at the onset of each stolonization period. Spermatogenesis is marked by a short proliferative period followed by differentiation and spermiogenesis. The first ultrastructural signs of spermatogenesis were found in coelomic spermatogonia on day 10 of stolon formation. Spermatogonia are joined by intercellular bridges, which are maintained until the early spermatid stage. Synaptonemal complexes mark the onset of meiosis, which is apparently synchronized in the syncytial clusters of primary spermatocytes. Spermiogenesis occurs during the final 10 days of stolonization and a variety of stages is present within a single animal. All sperm mature by the time the stolon detaches. Acrosome formation and nuclear condensation are described in addition to the ultrastructure of mature sperm.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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