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  • 1
    Electronic Resource
    Electronic Resource
    An efficient method for purification of cuprozinc superoxide dismutase from bovine erythrocytes (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 673-676 
    Details
    ISSN: 1420-9071
    Keywords: Cu,Zn-superoxide disumatase ; purification ; bovine erythrocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Cuprozinc superoxide dismutase (Cu,Zn-SOD) was isolated from bovine erythrocytes by pH-controlled ammonium sulfate-methanol extraction (ASME extraction). Adjustment of the pH of a suspension of the lysed red cells in the presence of ammonium sulfate (90% saturation) to pH 5.0, followed by partition with an equal amount of methanol, resulted in isolation of the enzyme with specific, activity of greater than 2000 units/mg of protein. Further purification using DEAE-cellulose column chromatography gave a highly purified Cu,Zn-SOD showing a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Using this procedure about 14 mg of pure Cu,Zn-SOD with a specific activity of 4728 units/mg of protein can be recovered from one liter of bovine blood. The enzyme was characterized and the results obtained were in agreement with earlier reports. This procedure appears, therefore, to be a convenient method for isolating the enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952871
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Prediction method of sand-movement direction in rivers by geochemical elements (1996)
    Springer
    Environmental geology 28 (1996), S. 88-98 
    Details
    ISSN: 1432-0495
    Keywords: Key words River sand ; Geochemical elements ; X-ray fluorescent spectroscopy ; Correlation number ; Ratio matching ; Correlation matrix ; Geological characteristics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  The analysis of sand samples by X-ray fluorescent spectroscopy (XRF) gives the ratio of the geochemical elements to construct the sand samples. The application of the ratio matching to sand samples represents the correlation number between two sand samples with respect to geochemical elements. When the correlation number between two sand samples is low, the two samples are not geochemically similar each other. This denotes that the exchange of sand between two sampling points is scarce or the two samples are independent. When the correlation number between two sand samples is high, the two samples are geochemically similar, signifying that the exchange of sand between two sampling points is frequent or there is sand movement between two sampling points. If there exists prominent sand movement in the study area, the correlation number is almost 1 and kilo count number per second of each geochemical element per weight decreases along the flow direction. The decrease is caused by the reduction of sizes and the adherence of dirt on the surface of sand particles. Since the flow direction in rivers is usually the same as the direction of sand movement, it can be verified. This study obtains satisfactory results applying the method of prediction in sand movement to sediments in rivers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002540050081
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    Paper (German National Licenses)
    Fulltext
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