ISSN:
1432-2048
Keywords:
Gibberellin and protoplast swelling
;
Phytochrome
;
Protoplast (swelling)
;
Triticum (protoplast swelling)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by far-red light over two light cycles, indicating the involvement of phytochrome. At 4°C, escape from reversibility occurred between 2 and 5 min after the exposure to red light. In exposure-response experiments, 20 s red light at 27 μmol m-2s-1 was sufficient to saturate the response. Exogenous gibberellic acid added in darkness in the presence of KCl also induced protoplast swelling. Gibberellins may act as an intermediate in the phytochrome-induced swelling of protoplasts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00397734
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