ISSN:
1432-2242
Keywords:
Gliadin
;
Two-dimensional electrophoresis
;
F1 monosomic analysis
;
chromosomal assignment
;
wheat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The gliadin components from four bread wheat cultivars: Chinese Spring, Capelle Desprez, Holdfast and Pane-247 and their monosomic F1s for the chromosomes of homoeologous groups 1 and 6 have been analyzed by two-dimensional (2-pH) polyacrylamide gel electrophoresis. Chromosomal location of gliadin genes and the allelic differences were well established by analyzing the different F1 monosomic hybrids, electrophoretical patterns and differences in relative staining intensity. A new gliadin encoded by a gene located on chromosome 6B in Chinese Spring is described. The two-dimensional patterns of gliadin in the other three varieties and the chromosomal location of their genes are reported for the first time. Relationships between gliadins in the two-dimensional patterns and the traditional system for their nomenclature are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273684
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