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  • Articles: DFG German National Licenses  (4)
  • Glucose  (2)
  • Physical Chemistry  (2)
  • 1
    Electronic Resource
    Electronic Resource
    A method for the simultaneous measurement of insulin release and B cell membrane potential in single mouse islets of langerhans (1981)
    Springer
    Diabetologia 21 (1981), S. 470-475 
    Details
    ISSN: 1432-0428
    Keywords: Glucose ; B cell ; albino mouse ; membrane potential ; spike frequency ; insulin release ; islet perifusion ; islet of Langerhans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A method has been developed for the simultaneous measurement of insulin release and electrical activity in single micro-dissected mouse islets of Langerhans. The effects of D-glucose have been studied in individual islets. Each islet was exposed to 0, 5.6, 11.1, 16.7, 22.2, 27.8 and 33.3 mmol/l glucose in a stepwise fashion. The minimum glucose concentration required to elicit spike activity is lower than that required to stimulate insulin release above basal levels and the maximum spike frequency occurs at a lower glucose concentration than does maximum insulin release. Following a reduction in glucose from 27.8 (or 33.3) to 5.6 mmol/l, membrane potentials returned to resting values within 2 min whereas insulin returned to basal values after 20 min. Increasing glucose from 5.6 to 27.8 mmol/l induced spike activity within 10 s; the insulin response was detected within 40 s. Thus, it is possible to use the single mouse islet for simultaneous measurements of insulin release and electrical activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257788
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of glucose at a rotating gold composite electrode by pulsed amperometric detection (1996)
    Weinheim : Wiley-Blackwell
    Electroanalysis 8 (1996), S. 443-446 
    Details
    ISSN: 1040-0397
    Keywords: Glucose ; Composite electrodes ; Pulsed amperometry ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Segregated composite electrodes mimic microelectrode ensembles. In this preliminary report, the use of a rotating gold-polychlorotri- fluoroethylene (or Kel-F, a 3M Company polymer) composite electrode in combination with pulsed amperometric detection (PAD) is described for the detection of glucose. Comparisons are made with results obtained at a solid gold disk electrode. The composite electrode exhibits a higher signal and a lower background than does the solid gold electrode. In terms of current density, the enhancement of the signal above the background is over 3-fold, similar to that observed with segregated graphite composite electrodes used in a constant potential mode. Little or no glucose signal is observed at either the solid gold or the gold composite electrode when employed in the constant potential mode. In the PAD mode, the signal is stable for periods in excess of an hour.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elan.1140080508
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Kinetics and mechanism of the Cl + CO reaction in air (1996)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 28 (1996), S. 763-771 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Long-path FTIR spectroscopy was used to study the kinetics and mechanism of the reaction of Cl atoms with CO in air. The relative rate constants at 298 K and 760 torr for the forward direction of the reaction of Cl with 13CO and the reaction of Cl13CO with O2 were k1 = (3.4 ± 0.8) × 10-14 cm3 molecule-1 s-1 and k2 = (4.3 ± 3.2) × 10-13 cm3 molecule-1 s-1, respectively (all uncertainty limits are 2σ). The rate constant for the net loss of 13CO due to reaction with Cl in 1 atm of air at 298 K was kCl+COobs = (3.0 ± 0.6) × 10-14 cm3 molecule-1 s-1. The only observed carbon-containing product of the Cl + 12CO reaction was 12CO2, with a yield of 109 ± 18%. Our results are in good agreement with extrapolations from previous studies. The reaction mechanism and the implications for laboratory studies and tropospheric chemistry are discussed. © 1996 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.7
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  • 4
    Electronic Resource
    Electronic Resource
    Rate constant for the reaction of Cl with CHCl3 (1996)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 28 (1996), S. 399-404 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The rate constant for the reaction of Cl with CHCl3 was measured relative to the Cl reaction with CH4 and CH3Cl using long path FTIR. The measured rate constant at 298 K and 1 atm total pressure was (1.21 ± 0.13) × 10-13 cm3 molecule -1s-1 (2σ) about 60% larger than the currently recommended value (although within the large error bars). The implications of the Cl + CHCl3 reaction for relative rate studies chloromethane reactivity, and tropospheric chemistry are discussed. © 1996 John Wiley & Sons, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.1
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    Paper (German National Licenses)
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