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A method for the simultaneous measurement of insulin release and B cell membrane potential in single mouse islets of langerhans (1981)

Scott, A. M. ; Atwater, I. ; Rojas, E.

Springer

Diabetologia 21 (1981), S. 470-475

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ISSN: 1432-0428

Keywords: Glucose ; B cell ; albino mouse ; membrane potential ; spike frequency ; insulin release ; islet perifusion ; islet of Langerhans

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary A method has been developed for the simultaneous measurement of insulin release and electrical activity in single micro-dissected mouse islets of Langerhans. The effects of D-glucose have been studied in individual islets. Each islet was exposed to 0, 5.6, 11.1, 16.7, 22.2, 27.8 and 33.3 mmol/l glucose in a stepwise fashion. The minimum glucose concentration required to elicit spike activity is lower than that required to stimulate insulin release above basal levels and the maximum spike frequency occurs at a lower glucose concentration than does maximum insulin release. Following a reduction in glucose from 27.8 (or 33.3) to 5.6 mmol/l, membrane potentials returned to resting values within 2 min whereas insulin returned to basal values after 20 min. Increasing glucose from 5.6 to 27.8 mmol/l induced spike activity within 10 s; the insulin response was detected within 40 s. Thus, it is possible to use the single mouse islet for simultaneous measurements of insulin release and electrical activity.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00257788

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Detection of glucose at a rotating gold composite electrode by pulsed amperometric detection (1996)

Elliott, Peter T. ; Olsen, Scott A. ; Tallman, Dennis E.

Weinheim : Wiley-Blackwell

Electroanalysis 8 (1996), S. 443-446

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ISSN: 1040-0397

Keywords: Glucose ; Composite electrodes ; Pulsed amperometry ; Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: Segregated composite electrodes mimic microelectrode ensembles. In this preliminary report, the use of a rotating gold-polychlorotri- fluoroethylene (or Kel-F, a 3M Company polymer) composite electrode in combination with pulsed amperometric detection (PAD) is described for the detection of glucose. Comparisons are made with results obtained at a solid gold disk electrode. The composite electrode exhibits a higher signal and a lower background than does the solid gold electrode. In terms of current density, the enhancement of the signal above the background is over 3-fold, similar to that observed with segregated graphite composite electrodes used in a constant potential mode. Little or no glucose signal is observed at either the solid gold or the gold composite electrode when employed in the constant potential mode. In the PAD mode, the signal is stable for periods in excess of an hour.

Additional Material: 4 Ill.