ISSN:
1432-1890
Keywords:
Key words Ectomycorrhiza
;
Glutamine synthetase
;
Glutamate dehydrogenase
;
Laccaria laccata
;
Immunogold localization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Using antibodies raised against glutamine synthetase (GS) and NADP-glutamate dehydrogenase (NADP-GDH) from Laccaria laccata, we examined tissular localization of GS and NADP-GDH in symbiotic tissues of Douglas fir/L. laccata ectomycorrhizas by immunogold labeling. Thin sections of mycorrhizal roots were first treated either with an anti-GS- or anti-NADP-GDH-specific antibody and then with a colloidal gold marker. Both enzymes appeared to be cytoplasmic. Our results also indicated the presence of GS in some fungal cells in dense cytoplasmic patches. It also appeared that GS is more abundant than NADP-GDH. The distribution of these nitrogen-assimilating enzymes in the fungal Hartig net and the sheath did not differ significantly. No labelling was observed in host cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s005720050051
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