ISSN:
1432-0533
Keywords:
Glutathione S-transferase placental type
;
T9 glioma cells
;
Dibutyryladenosine 3′,5′-cyclic monophosphate
;
Allylisothiocyanate
;
Benzylisothiocyanate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The effect of dibutyryladenosine 3′,5′-cyclic monophosphate (dibutyryl cAMP) on the expression of glutathione S-transferase placental type (GST-P) was examined in rat glioma cell line using an immunohistochemical technique. Cultured T9 glioma cells were negative for GST-P activity under normal conditions. However, treatment with 1 mM dibutyryl cAMP produced GST-P expression in about 50% of the cells, as well as some morphological changes. The expression of GST-P was increased with addition of dibutyryl cAMP together with 1 μg/ml allyl isothiocyanate (AITC) or 0.1 μg/ml benzyl isothiocyanate (BITC). With these combinated treatments, almost all cultured cells showed a strong positive reaction for GST-P, although AITC or BITC alone elicited GST-P in only 5% of the cultured cells. The results of the present study indicate that dibutyryl cAMP causes functional as well as morphological differentiation of T9 glioma cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00294371
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