Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    The group IIB intron from the green alga Scenedesmus obliquus mitochondrion: molecular characterization of the in vitro splicing products (1991)
    Springer
    Current genetics 20 (1991), S. 495-502 
    Details
    ISSN: 1432-0983
    Keywords: Scenedesmus obliquus ; Green alga ; Group II intron ; Self-splicing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the presence of high molar salt concentrations, the mitochondrial group IIB intron (rI1) from the green alga Scendesmus obliquus is capable of splicing in vitro. After establishing the optimal conditions for RNA processing the in vitro splicing products were unequivocally identified in self-splicing experiments by Northern hybridization analysis employing 3′end-labelled RNAs or exon-and/or intron-specific probes. Finally, two trans-esterification products were identified by sequencing of the spliced RNA. From our data we conclude that the processing of group II introns from both algal and yeast mitochondria is preceded by identical consecutive trans-esterification steps. The predicted secondary and tertiary structure of intron rI1 of S. obliquus contains all the motifs necessary for optimal self-splicing and which are characteristic of other group IIB introns from different species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334778
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The intron of a plastid gene from a green alga contains an open reading frame for a reverse transcriptase-like enzyme (1989)
    Springer
    Molecular genetics and genomics 218 (1989), S. 257-265 
    Details
    ISSN: 1617-4623
    Keywords: Green alga ; Plastid petD gene ; Intron group II ; Reverse transcriptase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plastid (pt) and mitochondrial (mt) DNA were isolated from the unicellular green alga KS3/2, which is presumed to be a species of the genera Ankistrodesmus or Monoraphidium. The DNA species are characterized by their different densities (pt, 1.685 g/ml; mt, 1.695 g/ml), individual restriction patterns, and their respective sizes of 130 and 47 kb. Using an intronic sequence from fungal mitochondria as a hybridization probe, intron sequences from mtDNA and from ptDNA were identified. DNA sequencing of the cloned ptDNA intron revealed that the plastid gene for subunit IV of the cytochrome b 6 /f petD complex is interrupted by a group II intron of unusual length (3533 bp). This was confirmed by transcript analysis. The intron encodes an open reading frame (ORF) which shows significant homology with reverse transcriptase genes from various genetic elements. This discovery is unique for plastomes and indicates that introns, retrotransposons, insertion elements and retroviruses may have a common evolutionary origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331276
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...