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  • 1
    ISSN: 1432-203X
    Keywords: Key words Cucumis sativus ; Plasma membrane ; H+-ATPase ; H+-transport rate ; Root temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cucumber plants (Cucumis sativus L.) were grown at different root temperatures and the H+-ATPase activities in the root plasma membranes were investigated. Electrolyte leakages and the amount of water transported from root to shoot indicated the sensitivity of cucumber root systems to low root temperature. The increase in H+-ATPase activity after 1 day of low root temperature treatment was reversed and gradually diminished as root temperatures of 10 °C continued for another 6 days. The H+-transport rate of the plasma membranes and the transcriptional activity of the corresponding gene expression closely followed the H+-ATPase activity. The role of H+-ATPase in root plasma membranes in terms of the root acclimation process at low root temperature is discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 84 (1992), S. 825-828 
    ISSN: 1432-2242
    Keywords: Aroma ; Rice (Oryza sativa) ; RFLP markers ; Grain quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report here the identification of a DNA marker closely linked to a gene for aroma in rice. The DNA marker was identified by testing 126 mapped rice genomic, cDNA, and oat cDNA, clones as hybridization probes against Southern blots, consisting of DNA from a pair of nearly isogenic lines (NILs) with or without the aroma gene. Chromosomal segments introgressed from the donor genome were distinguished by RFLPs between the NILs. Linkage association of the clone with the gene was verified using an F3 segregating for aroma. Cosegregation of the scented phenotype and donor-derived allele indicated the presence of linkage between the DNA marker and the gene. RFLP analysis showed that the gene is linked to a single-copy DNA clone, RG28, on chromosome 8, at a distance of 4.5 cM. The availability of a linked DNA marker may facilitate early selection for the aroma gene in rice breeding programs.
    Type of Medium: Electronic Resource
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