ISSN:
1573-7365
Keywords:
Huntington disease
;
kainic acid
;
receptors
;
glutamic acid
;
leukocytes
;
HPLC
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A factor from mammalian and human brain, which inhibits the rate of migration of leukocytes obtained from sufferers from Huntington disease (Walls and Ruwoldt, 1984), inhibited the specific binding of the neurotoxin [3H]kainic acid to rat brain synaptic membranes. The factor was present in sucrose-particulate but not in soluble fractions from rat sub-cortical tissue, and was destroyed by tryptic digestion. Whereas an ammonium sulfate fraction of direct saline extracts of brain (Walls and Ruwoldt, 1984) gave poor chromatography on HPLC, prior separation of a sucrose-particulate fraction resulted in much improved chromatography. There was a good concordance between leukocyte migration inhibitory activity and [3H]kainic acid binding inhibitory activity. The factor may be an endogenous modulator of the kainic acid subset of receptors for the excitatory neurotransmitter glutamic acid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00996921
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