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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 19 (2000), S. 1021-1026 
    ISSN: 1432-203X
    Keywords: Key words Atropa belladonna ; Hairy root ; Littorine ; Root growth ; Tropane alkaloid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Aseptically propagated regenerates were cultivated in a hydroponic apparatus, a phytotron or in the field, and their growth and littorine contents were investigated. No littorine was detected in aseptic regenerates cultured on solidified Murashige and Skoog medium, nor was it found in leaves under the three conditions tested. In roots, it was common features to all three conditions tested that littorine increased dramatically after transplantation from culture tubes and was a major alkaloid up to week 4; subsequently the littorine contents varied depending on the cultivation conditions. Roots cultivated in the field showed a marked thickening and rapid disappearance of littorine; those cultivated in the hydroponic apparatus were thin and maintained a high level of littorine for a long time. In a plant cultivated for 16 weeks in a pot, littorine content in the roots decreased with increasing root diameter.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 18 (1998), S. 249-251 
    ISSN: 1432-203X
    Keywords: Key wordsAtropa belladonna ; Hairy root ; Littorine ; Root culture ; Tropane alkaloid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A hairy root clone (M8) of Atropa belladonna, producing high levels of tropane alkaloids, was established by transformation with Agrobacterium rhizogenes (MAFF 03-01724). Littorine, an intermediate of tropane alkaloids, was detected by high-performance liquid chromatography and gas chromatography-mass spectrometry in the alkaloid fraction of the hairy roots and identified by nuclear magnetic resonance analysis. Littorine was also detected in the non-transformed root culture of A. belladonna.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-203X
    Keywords: Key words Forskolin ; Coleus forskohlii ; Hairy root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy roots of Coleus forskohlii were induced by infection with the Agrobacterium rhizogenes MAFF 03-01724 strain. Growth and forskolin production of two hairy root clones cultured in various liquid media were examined. Hairy root clone B9 grew well in woody plant liquid medium and showed a high forskolin yield (ca. 1.3 mg/ 100 ml flask) after 5 weeks of culture. The time course of growth and forskolin production of the clone B9 cultured in woody plant liquid medium was also examined. Rapid growth started at week 2 and continued until week 5. The highest forskolin yield (ca. 1.6 mg/100 ml flask) was obtained at week 5. Productivity was much higher than that previously reported.
    Type of Medium: Electronic Resource
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