ISSN:
1432-2242
Keywords:
Cultured plant cells
;
Mutants
;
Pyrimidine analogues
;
Haplopappus gracilis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Strains of Haplopappus gracilis (Nutt.) Gray cells resistant to 6-azauracil have been isolated from cultures of diploid cells. These strains are also resistant to 8-azaguanine, as is their parent. The variants are 100- to 125-fold more resistant to 6-azauracil than their parent, and they exhibit different spectra of cross resistance to other pyrimidine analogues. The phenotype of each variant is stable in the absence of selection. The majority of cells in cultures of the variants are diploid; all others examined were tetraploid. Initial rates of uptake of uracil are not reduced in the variants. Fluorouracil, to which two variants are resistant, is taken up by one of them as well as by the parent. Responses of the other two to fluorouracil are not correlated with decreased ability to accumulate this analogue.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00265474
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