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  • Digitale Medien  (3)
  • Daphnia  (1)
  • Flux Inhibition  (1)
  • Hemoglobin  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Pflügers Archiv 358 (1975), S. 275-288 
    ISSN: 1432-2013
    Schlagwort(e): Skeletal Muscle ; Electrolytes ; Potassium ; Sodium ; Flux Inhibition ; Membrane Stabilization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The membrane stabilizer diphenylhydantoin (DPH) did not alter the net content of Na, K, Ca or Mg in frog sartorius muscle freshly incubated or actively transporting Na and K following Na-loading and K-depletion. Resting influx of K from normal Ringer was significantly reduced by DPH, and this inhibition occurred in the ouabain-insensitive K-uptake. Inhibition of K-influx by DPH was overcome when [K]0 was raised to 10 mM, and Rb-influx was not sensitive to the inhibitor in 2.5 mM Rb-Ringer. Efflux of tracer K was reduced by DPH in the presence and absence of ouabain. Exchange of muscle Na was not affected under condit in which K-exchange was significantly reduced, but DPH appeared to cause increased net loss of Na from muscles washed in Na-free medium. The inhibition by DPH of resting K-exchange was not sensitive to wide variations in [Na]0 or in [Ca]0. The results suggest that the effect of DPH on frog skeletal muscle in normal ionic environment is to reduce the resting, passive component of K-exchange across the fibre membrane. This effect is discussed in relation to the membrane stabilizing actions of diphenylhydantoin.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-5117
    Schlagwort(e): Daphnia ; diel cycle ; excretion ; migration ; phosphorus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The timing and magnitude of diel migration in two daphnid assemblages were determined from a series of vertical profiles of daphnid size distribution. Animals were collected concurrently for gut fullness determination. Only large daphnids (〉 1.4 mm) migrated, but these animals could account for substantial vertical and diel differences in phosphorus excretion rate. Gut fullness measurements and time courses of diel vertical migration suggested that large Daphnia can cause a net downward flux of phosphorus during summer in thermally stratified lakes.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    The protein journal 10 (1991), S. 495-501 
    ISSN: 1573-4943
    Schlagwort(e): Hemoglobin ; recombinant hemoglobin ; protein folding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract A plasmid analogous to the one described by Nagai and Thogersen (Nature,309, 810–812, 1984) has been constructed for the expression of globins inE. coli. Induction with nalidixic acid produces high yields of a fusion protein, NS1-FX-β-globin, where NS1 represents 81 residues of a flu virus protein and FX represents a blood-clotting Factor Xa recognition sequence, Ile-Glu-Gly-Arg. This fusion protein is readily solubilized in 50 mM NaOH and remains in solution when thepH is adjusted to 8.6. Under these conditions, the fusion protein is hydrolyzed by activated Factor X, giving authentic β-globin which can be folded in the presence of cyanohemin and native α-chains to produce a tetrameric hemoglobin with the functional properties of natural human hemoglobin.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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